What is the role of elongation factor 1?

Elongation factor 1-alpha 1 (eEF1a1) is a protein that in humans is encoded by the EEF1A1 gene. This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome.

Is EF1A a strong promoter?

In human T cells EF1a (1.2kb version) is a strong and stable promoter (Michael C. Milone, Molecular Therapy, 2009). If it is not too big for your expression plasmid, it might be a safer choice.

What is CBA promoter?

The cytomegalovirus (CMV) early enhancer/promoter and the hybrid CMV enhancer/chicken β-actin (CBA) promoter are commonly used in gene transfer studies with both therapeutic and reporter genes. These promoters are typically used to provide robust, long-term expression in all cell types.

Is CMV promoter constitutive?

A variety of cellular and viral constitutive promoters have been used in expression vectors to introduce exogenous genes into cells. These promoters have distinct advantages; for example, cytomegalovirus (CMV) early enhancer/promoter directs a high level of transient gene expression in many types of cells [1]–[4].

What is the function of elongation factor 1 alpha 1?

Elongation factor 1-alpha 1 ( eEF1a1) is a protein that in humans is encoded by the EEF1A1 gene. This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome.

Where does the EF-1 alpha promoter come from?

The EF-1 alpha promoter, which offers a broad host range, is derived from the human EEF1A1 gene that expresses the alpha subunit of eukaryotic elongation factor 1 (Uetsuki et al. 1989; Wan Kim et al.

Which is the first intron of the elongation factor?

The human elongation factor 1 alpha (EF-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter J Biotechnol.

Which is elongation factor enhances the expression of foreign genes?

The human elongation factor 1 alpha (EF-1 alpha) first intron highly enhances expression of foreign genes from the murine cytomegalovirus promoter