What is dolichos Biflorus used for?
biflorus is used in the treatment of piles, pain, constipation, wounds, urinary calculi, cough, edema, asthma, etc.
What is lectin dolichos Biflorus?
General description. Lectin from Dolichos biflorus (horse gram) is a heterotetrameric glycoprotein with a molecular mass of around 113 kDa. Dolichos biflorus agglutinin (DBA) is specific for terminal α-linked N-acetyl-D-galactosamine (GalNAc). It is a dietary lectin.
What does dolichos Biflorus react with?
The extract of the seeds from Dolichos biflorus is virtually specific for A1 antigens on red blood cells. Warning: Contains sodium azide. Sodium azide may react with lead and copper plumbing to form highly explosive metal azide. On disposal, flush with a large volume of water to prevent azide buildup.
How do lectins cause agglutination?
Lectins bind specifically to the sugar residues, which project from red cell membrane causing RBCs to clump. This binding is as specific as antigenic recognition by antibodies.
Which blood group has the least amount of H antigen?
type AB
If a person has blood group O, the H antigen remains unmodified. Therefore, the H antigen is present in the highest amounts in blood type O and in the least amounts in blood type AB.
Which of the following lectins will agglutinate A1 antigens?
Anti-A1 Lectin
Anti-A1 Lectin This reagent will cause agglutination of red blood cells that carry the A1 antigen.
Which lectin Agglutinates B red cells?
The anti-A1 lectin reagent is used to differentiate A1 red blood cells from other subgroups of the A blood type. This reagent will cause agglutination of red blood cells that carry the A1 antigen.
What is the anti H reagent?
The Anti-H Lectin is a purified extract from seeds of Ulex europaeus. The reagent contains a phytohaemagglutinin which is virtually specific for the H antigen on human red blood cells.
What is the source of anti-A1?
Dolichos biflorus
This anti-A1 reagent is an extract from the seeds of Dolichos biflorus and can be used to differentiate A1 human red cells from other subgroups of A when tested by the tube technique.
What is the source of anti H lectin?
Anti-H Lectin The H antigen is part of the Hh system and is present in all red blood cells apart from the Oh (hh) phenotype, which is extremely rate. The anti-H lectin is made from an extract of Ulex europaeus seeds, diluted with a sodium chloride solution containing bovine albumin.
What is the molecular weight of Dolichos biflorus agglutinin?
Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size. This lectin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition…
What is the use of Dolichos biflorus ( DBA )?
Dolichos Biflorus (Horse Gram) Agglutinin (DBA), fluorescein (FITC), is a bright-green DBA-fluorophor conjugate. DBA has been used to establish secretor status in blood-group-A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.
What kind of lectin is Dolichos biflorus?
Dolichos biflorus is the most famous of these lectins. It is most often used to distinguish the two main subgroups of blood group A: A 1 and A 2.