What is cell staining buffer?

Cell Staining Buffer is an antibody diluent and cell wash buffer optimized for use in immunofluorescent staining of viable or fixed single cell suspensions. Cell Staining Buffer contains bovine calf serum as a protein carrier to reduce non-specific binding of antibodies and fluorochrome reagents to target cells.

What is in flow cytometry staining buffer?

This Flow Cytometry Staining Buffer is a buffered saline solution containing fetal bovine serum and sodium azide (0.09%) as a preservative. This buffer can be used for antibody and cell dilution steps, as well as all the wash steps required for the surface staining and flow cytometric analysis.

Why use staining buffer?

Staining buffer has been optimized for use when staining both live and fixed cells with antibodies. The presence of bovine calf serum in this product reduces background staining to target cells. Staining buffer also contains sodium azide to minimize receptor capping.

How do cells make staining buffers?

Cell Staining Buffer Recipe

1. Dissolve 8g of NaCl, 0.2g of KCl, 1.44g of Na2HPO4, and 0.24g of KH2PO4 in 800ml distilled H2O.
2. Add 20 ml of heat inactivated FBS.
3. Add 0.9 grams of sodium azide.
4. Adjust pH to 7.4 with HCl.
5. Adjust volume to 1L with additional distilled H2O.
6. Sterilize by filtration.
7. Store at 4oC.

How does FACS buffer work?

Flow Cytometry Staining Buffer (FACS Buffer) The buffer contains sodium azide as preservative and animal serum proteins (FBS/BSA) to help minimizing non-specific binding of antibodies. Note: NaN3 is added as a preservative. Use the buffer without NaN3 if you want to do functional assays with bacterial cells.

What is the main purpose of the flow cell?

The fluidics of the flow cytometer. The flow cell (also called the flow chamber) allows the cells (particles) within the samples to line up single file. This is a critical step for single-cell analysis.

What are the three main components of flow cytometry?

The three main components of a flow cytometer are the fluidics, optics, and electronics (Figure 1).

What is the UV intensity of nucblue live cell stain?

In most cases, staining intensity increases with time if cells are not washed prior to imaging. • NucBlue® Live Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm.

Why do we use detergent as a protein buffer?

The buffer uses detergent-based lysis, eliminating the need for mechanical cell disruption, providing a milder and easier alternative when isolating proteins from cell cultures. The formulation helps retain the protein structure and function needed for enzyme assays or immunoassays.

What can cell lysis buffer be used for?

Order requests are processed and shipped by your local distributor. Learn more Cell Lysis Buffer is a ready-to-use lysis buffer for use in ELISA and western blotting applications for total protein extraction from mammalian cells.

What kind of buffer is used for SDS-PAGE?

NuPAGE LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent. See all available buffers and reagents available for SDS-PAGE.