What is the Km value of hexokinase and glucokinase?
10 Difference between Hexokinase and Glucokinase
| Hexokinase | Glucokinase (Hexokinase D) |
|---|---|
| Hexokinase has high Km value that is high affinity for the substrate glucose | Glucokinase has high Km value that means low affinity for the substrate |
Why does hexokinase have low Vmax?
Hexokinase has a low Km and low Vmax, which means that it has a high affinity for glucose (it will bind easily to glucose molecules it encounters) but a low v-max, so even during fasting, when glucose levels are low, it is saturated.
What is the difference between hexokinase and glucokinase due to it Km 0.1 and 10?
The main difference between hexokinase and glucokinase is that the hexokinase is an enzyme present in all cells whereas the glucokinase is an enzyme only present in the liver. Furthermore, hexokinase has a high affinity towards glucose while glucokinase has a low affinity towards glucose.
What is glucokinase km?
Glucokinase is one of four glucose phosphorylating enzymes present in rat liver. Its distinctive features are a high K-m for glucose (high-K-m isozyme) and a rather narrow substrate specificity.
What is Km value?
The Michaelis constant (KM) is defined as the substrate concentration at which the reaction rate is half of its maximal value (or in other words it defines the substrate concentration at which half of the active sites are occupied).
What is difference between glucokinase and hexokinase?
What is hexokinase vs glucokinase?
Glucokinase and Hexokinase are enzymes which phosphorylate glucose to glucose-6-phosphate, trapping glucose inside the cell. Hexokinase is found in most tissues. Compared to glucokinase, hexokinase has a lower Km (higher binding affinity) and lower Vmax (lower capacity).
What does Vmax mean?
maximum
The rate of reaction when the enzyme is saturated with substrate is the maximum rate of reaction, Vmax. This is usually expressed as the Km (Michaelis constant) of the enzyme, an inverse measure of affinity. For practical purposes, Km is the concentration of substrate which permits the enzyme to achieve half Vmax.
What is the function of glucokinase?
Glucokinase (GCK) is a gene which plays an important role in recognising how high the blood glucose is in the body. It acts as the “glucose sensor” for the pancreas, so that when the blood glucose rises, the amount of insulin produced also increases.
Is glucokinase a hexokinase?
Glucokinase (GK) belongs to the hexokinase family (Grossbard and Schimke, 1966). It catalyzes the phosphorylation of a glucose molecule to produce glucose 6-phosphate and plays an important role in glucose utilization and metabolism in the liver and pancreas (Al-Hasani et al., 2003).
What is a Km value?
What is difference between hexokinase and glucokinase?