What is the substrate for HRP?

Colorimetric substrates for ELISA applications

What is OPD chemical?

o-Phenylenediamine (OPD) is an organic compound with the formula C6H4(NH2)2. This aromatic diamine is an important precursor to many heterocyclic compounds. It is isomeric with m-phenylenediamine and p-phenylenediamine.

What is a substrate in ELISA?

Colorimetric substrates are commonly used in ELISA. The substrate is a crucial component for a successful ELISA assay. The choice of ELISA substrate depends on the enzyme used in the preceding detection step. Alkaline phosphatase (ALP) and horseradish peroxidase (HRP) require different substrates.

Why is horseradish peroxidase important?

In addition to biomedical applications, horseradish peroxidase is one of the enzymes with important environmental applications. This enzyme is suitable for the removal of hydroxylated aromatic compounds (HACs) that are considered to be primary pollutants in a wide variety of industrial wastewater.

What is the purpose of adding the HRP substrate?

HRP Conjugates Horseradish peroxidase (HRP) is an enzyme used to amplify signal in photometric assays by catalyzing the conversion of chromogenic or chemiluminescent substrates for the detection of targets such as proteins, carbohydrates, and nucleic acids.

How does the HRP substrate work?

Horseradish peroxidase (HRP) catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product and the release of light as a by-product of the reaction. HRP functions optimally at a near-neutral pH and can be inhibited by cyanides, sulfides and azides.

What is OPD substrate?

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492.

Is diphenylamine soluble in water?

The compound is a colorless solid, but commercial samples are often yellow due to oxidized impurities. Diphenylamine dissolves well in many common organic solvents, and is moderately soluble in water. It is used mainly for its antioxidant properties.

Is ELISA A luminescence?

ELISA assays are an extremely common way to detect biomolecules in complex samples. Most ELISA assays use absorbance as a detection method. In some cases, when the concentrations to be measured are extremely low, luminescence provides more sensitivity and is used instead of absorbance.

Why is horseradish peroxidase used in ELISA?

HRP is used in immunohistochemistry and ELISA because it generates colored compounds. For detection of an antigen or protein molecule, HRP substrates have been designed so that they will generate a chemiluminescent, chromogenic, or fluorescent signal upon oxidation.

Is HRP a chemiluminescence?

The HRP substrate luminol emits light at 428 nm; however, luminol emits light only weakly, so enhancers are added to the reaction to increase the signal. This enhancement of a luminol-based signal is commonly referred to as enhanced chemiluminescence (ECL). Long signal duration.

What does HRP stand for?

Human resource planning
Human resource planning (HRP) is the continuous process of systematic planning ahead to achieve optimum use of an organization’s most valuable asset—quality employees.

What is the absorbance of opd for HRP?

OPD (o-phenylenediamine dihydrochloride) is used to detect HRP and yields a water soluble yellow-orange reaction product. The reaction product has an absorbance maximum of 492 nm.

What are the different types of HRP substrates?

HRP Substrate Types. There are several different types of HRP substrates. The type of substrate used for detection depends on the particular assay and mode of detection. Chromogenic HRP substrates, which remain in solution and become colored after reaction with HRP, are often used for ELISAs and other colorimetric assays.

Is Thermo Scientific Pierce OPD a water soluble substrate?

Thermo Scientific Pierce OPD is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492nm by ELISA plate readers. OPD yields a water soluble yellow-orange product when reacted with peroxidase with an absorbance maximum of 492.

What kind of substrate is Pierce OPD tablet?

Protein Assays and Analysis Detection Method Colorimetric Substrate Properties Chemical Substrate Substrate Type HRP (Horseradish Peroxidase) Substrate Description Pierce OPD Tablets Product Type ELISA Substrate