What is Tetrathionate Broth used for?

Tetrathionate Broth is recommended for the selective enrichment method of isolating Salmonella typhi and other salmonellae from faeces, sewage, etc. Organisms which reduce tetrathionate, such as salmonellae, flourish in the medium whilst many faecal organisms are inhibited1.

How to make Tetrathionate Broth?

1. Dissolve 46 g of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minutes to completely dissolve the medium.
3. Cool to 45-50°C and add 20 mL of the Iodine-Potassium Iodide Solution to the prepared Tetrathionate Broth Base.
4. DO NOT REHEAT AFTER ADDING IODINE SOLUTION.

What is the main selective agent in tetrathionate broth?

Mueller Kauffman Tetrathionate Novobiocin Broth contains peptic digest of animal tissue and casein enzymic hydrolysate as sources of carbon, nitrogen, vitamins and minerals. Ox bile and brilliant green are selective agents, which inhibits gram-positive and most other gram-negative organisms.

Why is selenite broth used for salmonella?

Selenite-F Broth was devised by Leifson, who demonstrated that selenite was inhibitory for coliforms and certain other microbial species, such as fecal streptococci, present in fecal specimens, and thus, was beneficial in the recovery of Salmonella species.

What is nutrient broth?

Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients.

How do you make selenite cystine broth?

Suspend 19.01 grams in 1000 ml purified / distilled water. Add 4 grams of sodium hydrogen selenite (M1079B). Warm to dissolve the medium completely. Distribute in sterile test tubes.

What is brilliant green agar?

BD Brilliant Green Agar is a highly selective medium used for the isolation of salmonellae other than S. Typhi from feces and other clinical specimens. PRINCIPLES AND EXPLANATION OF THE PROCEDURE. Microbiological method.

How is tetrathionate produced?

5B); specifically, tetrathionate is produced when inflammatory oxygen radicals react with thiosulphate derived from the hydrogen sulphide produced by fermentative gut commensals (Winter & Baumler, 2011).

How do you prepare nutrient broth?

How to prepare nutrient broth?

1. Add 13g of nutrient broth powder (CM0001B) in 1L of distilled water.
2. Mix and dissolve them completely.
3. Pour them into the final containers (eg. conical flask)
4. Sterilize by autoclaving at 121°C for 15 minutes.

What is the difference between a broth and an agar?

The only difference between broth and agar media is that broths do not contain an agar component. We use broth tubes primarily for specific assays, or (rarely) for bacteria that will not form colonies on a solid surface. Unlike preparation of agar plates, tubes are prepared with media already in the incubation vessel.

Is selenite cystine broth selective?

Selenite Cystine Broth is used as a selective enrichment medium for the isolation of Salmonella from feces, foods, pharmaceutical articles, water and other materials of sanitary importance. This medium conforms with specifications of The United States Pharmacopeia (USP).