What is the specific restriction site for SmaI?
SmaI is a restriction endonuclease used to cut DNA at the recognition sequence 5′-CCC/GGG-3′, generating DNA fragments with blunt termini.
What type of ends does SmaI produce?
SmaI produces blunt-ended fragments whereas XmaI produces a 5´ extension.
Does SmaI produce blunt ends?
Cleavage with two restriction endonucleases that produce blunt ends. 3. Cleavage with two restriction endonucleases that produce compatible overhangs….Recleavable Blunt Ends.
| Enzyme | Ligated to | Recleaved by |
|---|---|---|
| SmaI (CCC/GGG) | BsrBI, MspA1I (CMG/CGG) | BsaJI, HpaII, NciI, ScrFI |
| AfeI, SfoI, FspI | AciI | |
| NaeI | HpaII, NciI, ScrFI |
What does SmaI mean?
SMAI
| Acronym | Definition |
|---|---|
| SMAI | Societe de Mathematiques Appliquees et Industrielles (French) |
| SMAI | Styrene Maleic Anhydride Imide (resin) |
Are the EcoRI ends sticky or blunt?
The ends of a molecule cut by EcoRI have an overhanging region of single stranded DNA, and so are sometimes called sticky-ends. On the other hand, EcoRV is an example of an enzyme that cuts both strands in exactly the middle of its recognition sequence, producing what are called blunt-ends, which lack overhangs.
Are all blunt ends compatible?
Inserts Can Be Ligated in Two Directions Blunt ends are universally compatible with other blunt ends, so the directionality you can achieve by using sticky ends with different overhang sequences is not possible.
When is the best time to use SMAI?
Description. Thermo Scientific SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 30°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme
What are the reaction conditions for XbaI restriction enzyme?
Learn more Thermo Scientific XbaI restriction enzyme recognizes T^CTAGA sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.
Which is E coli strain carries the Smai gene?
Working continuously to be worthy of that distinction, NEB strives to develop enzymes of the highest purity and unparalleled quality. An E. coli strain that carries the SmaI gene from Serratia marcescens (ATCC 49779).
What are the reaction conditions for Thermo Scientific SMAI?
Learn more Thermo Scientific SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 30°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.