How does HA tag work?
The HA (hemagglutinin) tag is derived from the human influenza virus HA protein. It is well characterized and is used extensively as a general antibody epitope tag. HA tag antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe.
What is HA sequence?
HA Tag / Hemagglutinin Tag Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
What is an epitope tag?
Epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant DNA techniques. The fusion gene is cloned into an appropriate expression vector for the experimental cell type and host cells are transfected.
How big is a V5 tag?
about 14 aa
The V5 tag is about 14 aa, which as far as I know should correspond roughly 1-1.5KDa difference in molecular weight.
How do you purify HA tag proteins?
How do you purify HA-tagged proteins? HA-tagged recombinant protein can be affinity purified directly from a cell culture lysate or supernatant. The HA-tagged protein binds to the HA-tag specific monoclonal antibody conjugated on an agarose gel.
How much chromatin to use for a HA tag?
Use 3 µg for 25 µg of chromatin. Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors.
Where does the HA tag come from in a protein?
HA-tag. The HA tag is derived from the HA-molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein.
What kind of Mab is HA tag 6e2?
Immunohistochemical analysis of paraffin-embedded COS cells, untransfected (left), HA-Akt3 transfected (middle) or HA-Estrogen Receptor transfected (right), using an Akt antibody (top), an Estrogen Receptor antibody (middle) or HA-Tag (6E2) Mouse mAb (bottom).
Which is the best antibody for HA tag?
HRP conjugated Goat anti rabbit IgG was used and TMB was the substrate. YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH. WB: 293FT cells transfected with 15kDa HA tagged Vpr (an HIV1 accessory protein). IP: Nuclear lysate of HEK-293T cells transiently expressing HA-tagged protein. ICC/IF: U-2 cells. Mouse olineu cells.
https://www.youtube.com/watch?v=mn75POWFQDY