What can Peptide mass fingerprint be used for?

Peptide mass fingerprinting (PMF) (also known as protein fingerprinting) is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF.

What is meant by Peptide mass fingerprinting?

Peptide Mass Fingerprinting (PMF), also known as mass fingerprinting, was developed in 1993. It is a high throughput protein identification technique in which the mass of an unknown protein can be determined. Mass means the molecular size of peptides.

What is the principle of protein fingerprinting?

Each protein has a unique peptide map and hence swerves as fingerprint for the protein. It involves the chemical or enzymatic treatment of a protein resulting in the formation of peptide fragments followed by separation and identification of the fragments in a reproducible manner.

What is the limitation of peptide mass fingerprinting?

Peptide mass fingerprinting (PMF) has been widely used to identify single purified proteins from single-stage MS data. Unfortunately, this technique is less accurate than the peptide sequencing method and cannot handle protein mixtures, which hampers the widespread use of PMF.

How does peptidemass calculate mass of a protein?

PeptideMass [references] cleaves a protein sequence from the UniProt Knowledgebase (Swiss-Prot and TrEMBL) or a user-entered protein sequence with a chosen enzyme, and computes the masses of the generated peptides. The tool also returns theoretical isoelectric point and mass values for the protein of interest.

How does a peptidemass work in UniProt?

PeptideMass. PeptideMass [ references] cleaves a protein sequence from the UniProt Knowledgebase (Swiss-Prot and TrEMBL) or a user-entered protein sequence with a chosen enzyme, and computes the masses of the generated peptides.

How can peptidemass be used to check for post translational modifications?

The tool also returns theoretical isoelectric point and mass values for the protein of interest. If desired, PeptideMass can return the mass of peptides known to carry post-translational modifications, and can highlight peptides whose masses may be affected by database conflicts, polymorphisms or splice variants. Instructions are available.