Where does NheI cut?

Thermo Scientific NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (isoschizomers: AsuNHI, BmtI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

What is NheI?

Description: NheI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the NheI gene from Neisseria mucosa heidelbergensis. NheI activity is blocked by some combinations of overlapping CpG methylation. NZYTech’s NheI is an enzyme that acts at 37 °C.

How do we name restriction enzyme?

The first three letters of a restriction enzyme’s name are abbreviations of the bacterial species from which the enzyme has been isolated (e.g., Eco- for E. coli and Hin- for H. influenzae), and the fourth letter represents the particular bacterial strain.

Which is the best FastDigest nhei restriction enzyme?

Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: AsuNHI, BmtI.

When to use Thermo Scientific nhei restriction enzyme?

Order requests are processed and shipped by your local distributor. Learn more Thermo Scientific NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (isoschizomers: AsuNHI, BmtI).

How are HF restriction enzymes different from native enzymes?

High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and performance in a single buffer ( rCutSmart ™ Buffer ). All HF-restriction enzymes come with Gel Loading Dye, Purple (6X) .

How is nhei enzyme inhibited by salt concentration?

Cleaves to leave a 5´ CTAG extension which can be efficiently ligated to DNA fragments generated by AvrII, SpeI or XbaI. Inhibited by salt concentrations > 100 mM. This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA.