Is Bacillus megaterium oxidase positive?

Penicillin-Susceptible, Oxidase-Negative, Nonhemolytic, Nonmotile Bacillus megaterium in Disguise of Bacillus anthracis.

How do you identify Bacillus megaterium?

megaterium are concave, smooth and milk white, while B. mycoides looks white, coarser with fine threads around the colony and the color is milk white. The cell morphology (Figure 3 and Table 1) shows that the cell is rod-shaped, gram positive and sporous. The Table 2 shows further characteristics of the two isolates.

Is Bacillus subtilis DNAse positive or negative?

1. Positive Control: Bacillus subtilis I. DNAse Test- The DNAse test tests the ability for the unknown bacterium to produce deoxyribonuclease, which hydrolizes DNA. The DNAse agar contains the nutrients for DNA and contains methyl green as an indicator.

Is Staphylococcus aureus catalase positive or negative?

Staphylococcus aureus is a gram positive, catalase and coagulase positive coccus and by far the most important pathogen among the staphylococci. It produces enzymes such as catalase which are considered to be virulence determinants.

Is bacillus aerobic or anaerobic?

Bacillus species are rod-shaped, endospore-forming aerobic or facultatively anaerobic, Gram-positive bacteria; in some species cultures may turn Gram-negative with age. The many species of the genus exhibit a wide range of physiologic abilities that allow them to live in every natural environment.

Is the Kia tube capable of detecting glucose fermentation?

KIA tubes are also capable of detecting the production of H 2 S. It is seen as a black precipitate (second picture from the right). Sometimes the black precipitate obscures the butt of the tube. In such cases, the organisms should be considered positive for glucose fermentation (yellow butt).

How to test for acetylmethyl carbinol in Bacillus cereus?

Test for production of acetylmethyl-carbinol by pipetting 1 ml culture into 16 × 125 mm test tube and adding 0.6 ml alpha -naphthol solution ( R89) and 0.2 ml 40% potassium hydroxide ( R89 ). Shake, and add a few crystals of creatine. Observe results after holding for 1 h at room temperature.

What kind of fermentation does Proteus mirabilis have?

Sometimes the black precipitate obscures the butt of the tube. In such cases, the organisms should be considered positive for glucose fermentation (yellow butt). Proteus mirabilis (pictured here, second from right) is a glucose positive, lactose negative, sulfur reducing enteric. This is a differential medium.