What are Cos-7 cells used for?

The COS-7 (CV-1 in Origin with SV40 genes) cells are known as non-steroidogenic cells because they are derived from kidney cells and the kidney is defined as a non-steroidogenic organ. Therefore, COS-7 cells are used for transfection experiments to analyze the actions of functional molecules including steroids.

Where can I find Cos-7 cells?

There are several well-established mammalian cell lines that have been used for heterologous expression of membrane proteins: the COS-7 cell line derived from the kidney of the African green monkey (Cercopithecus aethiops) (Yasumura and Kawakita, 1963), the CHO cell line derived from the ovaries of the Chinese hamster …

Can you transfect mammalian cells?

Plasmid transfection into mammalian cells is fairly straightforward and the resultant cells can either express the plasmid DNA transiently (similar to bacteria) or incorporate the genetic material directly into the genome to form a stable transfection.

What is stable transfection?

Stable transfection refers either to the permanent expression of the gene of interest through the integration of the transfected DNA into the nuclear genome, or the maintenance of a transfected plasmid as an extra chromosomal replicating episome within the cell.

What is stable transfection used for?

Stable transfection is mainly used for producing proteins on a large scale, studying gene expression, and the development of gene therapy treatments for diseases such as cancer.

What is stable gene expression?

Stable expression system can refer to a cell line that is simply stable, in that it will remain unchanged over a long time. A common method used is to co-transfect the gene of interest with another gene for antibiotic resistance, and treat the transfected cells with the antibiotic.

How are plasmids used to transfect mammalian cells?

( f) Plasmids are then used to transfect mammalian cells. Scale bar, 50 μm. To validate our system, we compared HEK293 cells transiently transfected with a MultiLabel plasmid encoding five fluorescent proteins with cells co-transfected with individual parental expression vectors.

Can a transiently transfected cell line express all proteins?

Transiently transfected mammalian cells homogenously express all proteins and it is possible to generate stable cell lines expressing multiple transgenes in a single step. The viability of transfected cells is not affected as they show, for instance, the expected behaviour after stimulation with growth factors.

How are transduced cells able to maintain stable expression?

When transduced cells are put under selection with the antibiotic G418, cell lines can be obtained at high frequency that stably maintain the expression cassettes of the vector DNA and exhibit stable, high-level expression of the reporter gene.

How is gene expression transient in mammalian cells?

Transduction of Mammalian Cells by BacMam1 GFP Virus. Cells were seeded in 6-well culture dishes at 150,000–250,000 cells per well. Culture medium was removed, replaced with virus inoculum, and incubated for 1 hr at 37°C. After removal of virus, fresh medium was added and cultures were incubated at 37°C.