How does Antimycin affect metabolism?

We conclude that rotenone and antimycin A inhibit the sodium-dependent transport of fluid, phosphate, and glucose by blocking mitochondrial ATP production. Furthermore, the inhibition of mitochondrial oxidative metabolism and the inhibition of net sodium transport are closely correlated.

What are the effects of antimycin A?

Antimycin A is an inhibitor of cellular respiration, specifically oxidative phosphorylation. Antimycin A binds to the Qi site of cytochrome c reductase, inhibiting the oxidation of ubiquinone in the Qi site of ubiquinol thereby disrupting the Q-cycle of enzyme turn over.

How does antimycin A affect cellular respiration?

Antimycin is widely used in research to study cellular respiration because of its potent ability to block the ETC. In addition to mitochondrial respiratory inhibition it has been shown to directly inhibit Bcl-2 related proteins. It is a carbon based, ring-containing compound that also contains oxygen and nitrogen.

What type of inhibitor is antimycin A?

electron transport chain
Antimycin is a potent electron transport chain (ETC) inhibitor. It inhibits the flow of electrons through complex III of the ETC by blocking the passage of electrons from cytochrome b to cytochrome c.

What mitochondrial enzyme is affected by antimycin A?

cytochrome bc1 complex
Antimycin A (antimycin), one of the first known and most potent inhibitors of the mitochondrial respiratory chain, binds to the quinone reduction site of the cytochrome bc1 complex.

How does antimycin A affect oxygen consumption?

Antimycin A at levels that abolish oxygen consumption had a slight, although statistically significant, inhibitory effect on the toxicity elicited by t-butylhydroperoxide in U937 cells. The protective effect was observed after 6 h of post-treatment incubation, but was no longer apparent after 24 h.

How do you dilute antimycin A?

Antimycin A1 is sparingly soluble in aqueous solutions. To enhance aqueous solubility, dilute the organic solvent solution into aqueous buffers or isotonic saline.

What does Antimycin a bind to?

Antimycin A (antimycin), one of the first known and most potent inhibitors of the mitochondrial respiratory chain, binds to the quinone reduction site of the cytochrome bc1 complex.

Is Antimycin a light sensitive?

Sensitivity of the electron transport to antimycin was highest at saturating light intensity and so was the difference between WT (20% inhibition) and mutant (50% inhibition). In antimycin-treated leaves, Fs values at steady state remained higher in mutants than in WT, whereas the qN values were comparable.

Is Antimycin an antibiotic?

—Antimycin A, a fungicidal antibiotic which specifically inhibits metabolic reduction of cytochrome c, was found to be lethal for Bacillus megaterium.

What are the physical properties of Antimycin?

Physical Properties of Antimycin A Antimycin A is a solid and its melting point has been experimentally determined to be 140 degrees Celsius. The compound’s color is white, and in its purest form is beautifully crystalline.

What is the molecular weight of Antimycin A?

Antimycin A PubChem CID 14957 Synonyms Antimycin A Antipiricullin Fintrol Viros Molecular Weight 548.6 Date s Modify 2021-06-12 Create 2005-06-01

How to calculate your active metabolic rate ( AMR )?

Active Metabolic Rate Calculator. Active Metaboilc Rate (AMR) is the actual amount of calories you burn each day due to physical activitites like going to the gym, aerobics, cooking, gardening, yoga and so on. Knowing your AMR is essential incase you want lose weight, stay fit and healthy or gain weight.

How is mitochondrial damage induced by antimycin A?

Here, we induced mitochondrial damage in human RPE cells (ARPE-19 and hRPE), using antimycin A, an inhibitor of complex III of the electron transport chain, and investigated cellular viability, mitochondrial structure and function, and autophagy activity.

What do you mean by active metaboilc rate?

Active Metaboilc Rate (AMR) is the actual amount of calories you burn each day due to physical activitites like going to the gym, aerobics, cooking, gardening, yoga and so on.