What is solid phase microextraction sampling?

Solid phase microextraction, or SPME, is a solid phase extraction sampling technique that involves the use of a fiber coated with an extracting phase, that can be a liquid (polymer) or a solid (sorbent), which extracts different kinds of analytes (including both volatile and non-volatile) from different kinds of media.

What is solid phase microextraction used for?

SPME is widely used for a variety of applications involving environmental, biological and pharmaceutical samples, foods and beverages, flavors and fragrances, forensics and toxicology and product testing. Typical uses include: Environmental analyses of water & air samples.

What is microextraction method?

Microextraction is defined as an extraction technique where the volume of the extracting phase is very small in relation to the volume of the sample, and extraction of analytes is not exhaustive. From: Journal of Chromatography A, 2000.

Why solid phase microextraction SPME is a powerful sample preparation tool prior to mass SPectrometric analysis?

SPME reduces the time necessary for sample preparation, decreases purchase and disposal costs of solvents and can improve detection limits. The SPME technique is ideally suited for MS applications, combining a simple and efficient sample preparation with versatile and sensitive detection.

What is liquid phase microextraction?

Liquid-phase microextraction is a miniaturized form of traditional liquid–liquid extraction in which the extracting organic phase is limited to a few microliters for extraction of target analytes.

Why is SPME used?

SPME and Environmental Analysis The SPME technique has been widely used for the analysis of environmental pollutants in air, water, soil and sediment samples, using on-site or off-site analytical approaches.

What is single drop microextraction?

Single-drop microextraction (SDME) is often used to represent the acceptor phase where only a small microdroplet (0.5–2.0 µl) is suspended on the tip of a microsyringe; while liquid–liquid microextraction (LLME) is used when the acceptor phase is >10 µl [2].

What is solid phase extraction technique?

Solid phase extraction (SPE) is a technique designed for rapid, selective sample preparation and purification prior to the chromatographic analysis (e.g. HPLC, GC, TLC). In SPE, one or more analytes from a liquid sample are isolated by extracting, partitioning, and/or adsorbing onto a solid stationary phase.

How is SPME?

SPME is a sampling technique based on absorption developed by Arthur and Pawliszyn. With SPME, the analytes are absorbed from the liquid or gaseous sample on to an absorbent coated fused silica fibre, which is part of the syringe needle, for a fixed time.

How do SPME fibers work?

SPME is a solvent-less extraction procedure that involves the exposure of a coated fused silica fiber to a gaseous or liquid sample or the headspace above a liquid or solid sample. By sampling the headspace above a complex sample matrix, SPME helps to minimize effects due to interfering organic compounds.